Textbook in PDF format

Method Development in Analytical HPLC presents the essential information for understanding the process of developing an HPLC method of analysis. It includes foundational information related to HPLC, as well as discussion of sample types, the properties of analytes and matrices in the samples, and sample preparation. The core of the book describes the best ways for approaching method development in various types of HPLC and the criteria for method optimization and validation. This book provides clear guidance for adopting analytical methods from the literature and describes the development of original methods with selection of the suitable type of HPLC, of specific columns, mobile phase, and detection techniques with an emphasis on the use of mass spectrometry for detection, as well as optimization and validation of the chosen analytical method. The book includes useful details on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, size exclusion, and chiral. Method Development in Analytical HPLC also includes information about green chemistry in analytical methods, computer assisted method development, and other key contemporary aspects of the subject. • Offers a systematic and logical presentation of the foundational of analytical HPLC. • Goes in-depth on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, and size exclusion. • Includes methods with an emphasis on the use of mass spectrometry for detection. Introductory concepts related to a chemical analysis Retention and elution processes in high-performance liquid chromatography Instrumentation in high-performance liquid chromatography Performance and utilization of high-performance liquid chromatography separation and detection Samples in high-performance liquid chromatography analysis Sample preparation for high-performance liquid chromatography analysis Chromatographic columns in high-performance liquid chromatography Mobile phase and the role of solvents in high-performance liquid chromatography Development of a high-performance liquid chromatography analytical method Selection of the best options in developing a high-performance liquid chromatography analytical method Method and data validation in high-performance liquid chromatography analysis