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Cell culture is cell cloning technology that simulates in vivo environment conditions such as asepsis, appropriate temperature, and pH as well as certain nutritional conditions to enable cells to survive, grow, reproduce, and maintain their structure and function. Cell culture can be used to grow human, animal, plant, and microbial cells. Each type of cell culture has its own characteristics and essential conditions. This book focuses on the advanced technology and applications of cell culture in the research and practice of medical and life sciences. Chapters address such topics as primary cancer cell cultures, 2D and 3D cell cultures, stem cells, nanotechnology, and more. A Brief Concept of Cell Culture: Challenges, Prospects and Applications Overview of Primary Cell Culture Models in Preclinical Research of Prostate and Bladder Cancer Two-Dimensional and Three-Dimensional Cell Culture and Their Applications Isolation and Expansion of Mesenchymal Stem/Stromal Cells, Functional Assays and Long-Term Culture Associated Alterations of Cellular Properties The Use of Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) to Study Ivermectin-Mediated Molecular Pathway Changes in Human Ovarian Cancer Cells Nanotechnology Application and Intellectual Property Right Prospects of Mammalian Cell Culture